Optimisation du rendement d'extraction des substances bioactives par procédé de macération à partir des racines de l'espèce végétale Rhamnus Alaternus.

Abstract

Rhamnus Alaternus, is a medicinal plant widely used in traditional medicine in Algeria, it has a strong chemical potential as a source of active ingredients, known for their many therapeutic virtues in traditional medicine. Our work consists in optimizing the extraction yield, by hydro-ethanolic maceration process (80%, V/V), of the bioactive substances, in this case, the poly-phenolic compounds contained in the roots of Rhamnus Alaternus. The experimental design methodology presented by Box-Behnken's design and explored by « Minitab 16 », confirmed that the parameters having a main and significant interactive effect on the extraction yield are the contact time and to a lesser degree the solid/liquid ratio. The stirring speed has a negligible effect on this extraction process. The mathematical model obtained, valid at 99.38%, allowed the optimum extraction to be achieved, namely: S/L ratio = 0.01/10 (g/ml); stirring speed = 839.38 rpm; time = 25 minutes, giving an extraction rate equal to 45.40%.